Tissue Culture

[mito0]

this may sound silly, but given all the unknowns surrounding tc and its future, i think it would actually be a good idea for everyone to keep good records about the source of their boo, especially through trading. in any trading community, it's easy to lose track of lineage, but plants with documented 'wild stock' lineage could eventually have more of a demand than 'tc stock' for any number of unforseen reasons. it's fairly certain at this point, for example, that practically every fargesia rufa in america is tc stock, but i'd be interested in finding someone who actually has a division from verified wild stock from which i could get a division.

[Jeff: Igor's Apprentice]

I would like to see this thread travel in the direction of what it would take to do it and perhaps get things moving if someone is interested in doing this as a personal project...

I had been hoping to find a ready made laminar flowhood, but my interest in making one from scratch has been piqued....


Here is a link from a mushroom site with instructions. There is a link to another site from an orchid enthusiast with instructions at the bottom of the page.

http://www.fungifun.org/English/Flowhood

One of the most important factors in tc is maintaining sterility. A pressure cooker and a laminar flow hood are the main tools in achieving that goal.

[Thuja]

The laminar flowhood looks like a good winter project. The hood could double as desk where you could read books and nap without fear of recurrent dust mite attacks.

[Jeff: Igor's Apprentice]

The laminar flowhood looks like a good winter project. The hood could double as desk where you could read books and nap without fear of recurrent dust mite attacks.

That may be just what my allergist ordered.

[Jeff: Igor's Apprentice]

Thuja, you anywhere near Milton, Wisconsin?

I just got a user group email from Dr. Stiff of Kitchen Culture Kits and she has just relocated her operation there.

[ocimum_nate]

Hoe_NC
Where did you get your Plants from test tubes book? I looked on amazon but found that most of the used books were just as expensive as the new. Anyhow I really want to try to do some tc stuff and have read about some low cost methods that have been used in India and other third world countries that I may be able to afford to try.

[Jeff: Igor's Apprentice]

I think I got it on Amazon.com. It was awhile ago.
I found about 6 copies just now on ebay- several were charging absolutely ridiculous shipping amounts, though. You might keep an eye on ebay though.

[Thuja]

Thuja, you anywhere near Milton, Wisconsin?
I just got a user group email from Dr. Stiff of Kitchen Culture Kits and she has just relocated her operation there.

I gotta check where that is exactly. I'll drive over and order a dish to go... [drum rimshot]

It seems like a large household HEPA air filter could be fitted with a homemade adapter so it would connect to a hood.

Another thought... Maybe check your local college for a horticulture course on micropropagation. That would give access to some good equipment for any allowed extracurricular experimentation.

[caricapapaya]

a laminar flow hood is ideal, but to get started you dont necessarily need one.

A still air cabinet can easily be made and can be very effective. There are links on the web describing them.

Most important is to get a working knowledge and some practical experience in sterile technique. Then it will become second nature.

I have had good success working in the open air with bacteria and fungi...cycles are much shorter and they grow a lot faster than plants. I wouldnt recommend it for plant tc, but some alcohol and a glove box (still air cabinet) would do the trick to start.

As was mentioned by Ocimum Nate, there are techniques used that are very good for hobbyists.

carica

[philinshelton]

Mito0, you might ask Ned at Bamboo Garden in Oregon, for non-TC rufa. I know he sold/sells lots of TC stock, but I would bet that he was selling conventionaly propagated plants before the TC rufa became available.

I hope that someone has success with TC bamboo for the hobbyist. Since there is so little information on the subject, I would like to see the ABS and/or chapters fund some research, and publish detailed protocols for a few genera.

[Jeff: Igor's Apprentice]

They are selling a laminar flowhood on ebay right now that was supposedly the original laminar flowhood used by Professor P. Fanaticus.

I had to research what that meant- being quite curious upon reading that (and new to the gourmet mushroom world), but lets just say, if true, it would be a real piece of cult history on the illicit mycology side. Can't figure out how it ended up in KY though since P. Fanaticus worked in the PNW. The feds busted up his operation. Do the feds have a paraphanalia redistribution center in Kentucky?

[Jeff: Igor's Apprentice]

I must have it!!!!!

Then- Lector's mask.

Then, I'll move downtown to the Biltmore Estate
(Actual set for Mason Verger's house in "Hannibal" )

[Jeff: Igor's Apprentice]

Thuja said:

The laminar flowhood looks like a good winter project. The hood could double as desk where you could read books and nap without fear of recurrent dust mite attacks.

check out this auction for a "laminar flow cleanroom":

http://cgi.ebay.com/LAMINAR-FLOW-CLEAN- ... dZViewItem

Are you one of those people who wake up night after night with bothersome allergies from the air we breath. I have the unit for you, a Laminar Flow Hepa Filter, large enough to put your bed into and have pure clean air all night long.

Act fast: bidding is only at $500 and ends in 2 days.

[bambooweb]

INBAR has a "Training Manual" for Micro-Propagation of Bamboo in their website. It is a 116 page pdf file at: http://www.inbar.int/publication/pubdet ... blicid=115
You can see the text there but to get the pdf file you need to be an INBAR Affiliate. The pdf file has a lot of color photos. The ABS in an INBAR Affiliate so ABS members can get the password from their chapter rep. or president.

Bill

[foxd]

Or you could just download it here:

http://www.inbar.int/publication/pdf/IN ... t_No27.pdf